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Tested Applications
| Positive FC detected in | PHA treated human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98324-3-RR targets SEMA7A in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SEMA7A fusion protein Eg2005 Predict reactive species |
| Full Name | semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) |
| Calculated Molecular Weight | 75kDa |
| GenBank Accession Number | NM_003612.5 |
| Gene Symbol | SEMA7A |
| Gene ID (NCBI) | 8482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75326-1 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane-associated glycosylphosphatidylinositol (GPI)-linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, SEMA7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SEMA7A antibody 98324-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
