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Anti-Mouse Ki-67 Rabbit Recombinant Antibody

Ki-67 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241768E2

Cat no : 98224-1-RR

Synonyms

Validation Data Gallery

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  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR)

    FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR

    1x10^6 untreated or Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR)

    FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR

    1x10^6 Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC (Intra) detected inCon A treated C57BL/6 mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98224-1-RR targets Ki-67 in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name antigen identified by monoclonal antibody Ki 67
Calculated Molecular Weight351kDa
GenBank Accession NumberNM_001081117.2
Gene Symbol ki67
Gene ID (NCBI) 17345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki-67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Protocols

Product Specific Protocols
FC protocol for Ki-67 antibody 98224-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols