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Anti-Human CD157 Rabbit Recombinant Antibody

CD157 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241424H5

Cat no : 98146-1-RR

Synonyms

BST 1, Bone marrow stromal antigen 1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2, ADP ribosyl cyclase 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD157 Rabbit Recombinant Antibody (98146-1-RR)

    FC experiment of human peripheral blood leukocytes using 98146-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.15 ug Anti-Human CD157 Rabbit Recombinant Antibody (98146-1-RR, Clone: 241424H5), or Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD157 Rabbit Recombinant Antibody (98146-1-RR)

    FC experiment of human peripheral blood leukocytes using 98146-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.15 ug Anti-Human CD157 Rabbit Recombinant Antibody (98146-1-RR, Clone: 241424H5) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98146-1-RR

    Affinity and Kinetic Characterization of 98146-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98146-1-RR against Human CD157 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98146-1-RR targets CD157 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name bone marrow stromal cell antigen 1
Calculated Molecular Weight 318 aa, 36 kDa
GenBank Accession NumberBC012095
Gene Symbol CD157
Gene ID (NCBI) 683
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores.

Protocols

Product Specific Protocols
FC protocol for CD157 antibody 98146-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols