FITC Plus Anti-Human CD157 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using FITC-98146

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul FITC Plus Anti-Human CD157 Rabbit RecAb (FITC-98146, Clone: 241424H5) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using FITC-98146

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul FITC Plus Anti-Human CD157 Rabbit RecAb (FITC-98146, Clone:241424H5)(red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD157 Uni-rAb^TM Recombinant Antibody for FC

Cat No. FITC-98146

Clone No.241424H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

BST 1, Bone marrow stromal antigen 1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2, ADP ribosyl cyclase 2

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98146 targets CD157 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: bone marrow stromal cell antigen 1
• Calculated Molecular Weight: 318 aa, 36 kDa
• GenBank Accession Number: BC012095
• Gene Symbol: CD157
• Gene ID (NCBI): 683
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q10588
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores.