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Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody

MCP-1/CCL2 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230483A9

Cat no : 98028-1-RR

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of RAW 264.7 cells using Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR)

    FC experiment of RAW 264.7 using 98028-1-RR

    1x10^6 unstimulated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of RAW 264.7 cells using Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR)

    FC experiment of RAW 264.7 using 98028-1-RR

    1x10^6 unstimulated (black) or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98028-1-RR

    Affinity and Kinetic Characterization of 98028-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98028-1 -RR against Mouse Mcp1 were performed. The affinity constant is below 1 nM.

Tested Applications

Positive FC (Intra) detected inLPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98028-1-RR is MCP-1/CCL2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name chemokine (C-C motif) ligand 2
GenBank Accession NumberNM-011333
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Protocols

Product Specific Protocols
FC protocol for MCP-1/CCL2 antibody 98028-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols