Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody, PBS Only

MCP-1/CCL2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230483A9

Cat no : 98028-1-PBS

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

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Validation Data Gallery

• 

  FC experiment of RAW 264.7 using 98028-1-RR (same clone as 98028-1-PBS)

  1x10^6 unstimulated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98028-1-PBS in a different storage buffer formulation.

• 

  FC experiment of RAW 264.7 using 98028-1-RR (same clone as 98028-1-PBS)

  1x10^6 unstimulated (black) or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98028-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98028-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98028-1 -RR against Mouse Mcp1 were performed. The affinity constant is below 1 nM.

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Product Information

The immunogen of 98028-1-PBS is MCP-1/CCL2 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: chemokine (C-C motif) ligand 2
• GenBank Accession Number: NM-011333
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 20296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.