CoraLite® Plus 647 Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody

MCP-1/CCL2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230483A9

Cat no : CL647-98028

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

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Validation Data Gallery

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  FC experiment of RAW 264.7 using CL647-98028

  1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL647-98028, Clone:230483A9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of RAW 264.7 using CL647-98028

  1x10^6 LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL647-98028, Clone:230483A9)(red) or Isotype Control (blue). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL647-98028, Clone:230483A9) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: LPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98028 is MCP-1/CCL2 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: chemokine (C-C motif) ligand 2
• GenBank Accession Number: NM-011333
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 20296
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.