Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of MCF-7 using 85113-4-RR
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of MCF-7 using 85113-4-RR
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ETS2 Recombinant antibody (85113-4-RR, Clone:242817H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ETS2 Recombinant antibody (85113-4-RR, Clone:242817H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Affinity and Kinetic Characterization of 85113-4-RR
Biolayer interferometry (BLl) kinetic assays of 85113-4-RR against Human ETS2 were performed. The affinity constant is below 1 pM.
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Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of MCF-7 using 85113-4-RR
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of MCF-7 using 85113-4-RR
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ETS2 Recombinant antibody (85113-4-RR, Clone:242817H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
AFFINITY Figures
Affinity and Kinetic Characterization of 85113-4-RR
Biolayer interferometry (BLl) kinetic assays of 85113-4-RR against Human ETS2 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ETS2 Recombinant antibody
Catalog Number
85113-4-RR
Citations
-
Dilutions
IF/ICC : 1:500-1:2000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet