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PBK/SPK Recombinant antibody

PBK/SPK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242545A11

Cat no : 84970-1-RR

Synonyms

PBK, MAPKK like protein kinase, Lymphokine-activated killer T-cell-originated protein kinase, EC:2.7.12.2, CT84

Filter:
  • Western Blot (WB) analysis of various lysates using PBK/SPK Recombinant antibody (84970-1-RR)

    WB analysis using 84970-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84970-1-RR (PBK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PBK/SPK Recombinant antibody (84970-1-RR)

    IF Staining of HeLa using 84970-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBK/SPK antibody (84970-1-RR, Clone: 242545A11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using PBK/SPK Recombinant antibody (84970-1-RR)

    FC experiment of HepG2 using 84970-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PBK/SPK Recombinant antibody (84970-1-RR, Clone:242545A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, L02 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84970-1-RR targets PBK/SPK in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PBK/SPK fusion protein Ag9033
Full Name PDZ binding kinase
Calculated Molecular Weight 322 aa, 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC015191
Gene Symbol PBK
Gene ID (NCBI) 55872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).

Protocols

Product Specific Protocols
WB protocol for PBK/SPK antibody 84970-1-RRDownload protocol
IF protocol for PBK/SPK antibody 84970-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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