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MCM3 Recombinant antibody

MCM3 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242103B9

Cat no : 84792-5-RR

Synonyms

P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM3 Recombinant antibody (84792-5-RR)

    WB analysis using 84792-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84792-5-RR (MCM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MCM3 Recombinant antibody (84792-5-RR)

    IHC staining of human colon cancer using 84792-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MCM3 Recombinant antibody (84792-5-RR)

    IHC staining of human colon cancer using 84792-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using MCM3 Recombinant antibody (84792-5-RR)

    FC experiment of HeLa using 84792-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MCM3 Recombinant antibody (84792-5-RR, Clone:242103B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of K-562 cells using MCM3 Recombinant antibody (84792-5-RR)

    FC experiment of K-562 using 84792-5-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug MCM3 Recombinant antibody (84792-5-RR, Clone:242103B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84792-5-RR

    Affinity and Kinetic Characterization of 84792-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84792-5-RR against Human MCM3 were performed. The affinity constant is 73.2 pM.

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Raji cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84792-5-RR targets MCM3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MCM3 fusion protein Ag7966
Full Name minichromosome maintenance complex component 3
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 102-105 kDa
GenBank Accession NumberBC001626
Gene Symbol MCM3
Gene ID (NCBI) 4172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).

Protocols

Product Specific Protocols
WB protocol for MCM3 antibody 84792-5-RRDownload protocol
IHC protocol for MCM3 antibody 84792-5-RRDownload protocol
FC protocol for MCM3 antibody 84792-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols