Tested Applications
Positive WB detected in | K-562 cells, Raji cells, HL-60 cells |
Positive IF/ICC detected in | Raji cells |
Positive FC (Intra) detected in | K-562 cells, HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84792-2-RR targets MCM3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | MCM3 fusion protein Ag7966 Predict reactive species |
Full Name | minichromosome maintenance complex component 3 |
Calculated Molecular Weight | 91 kDa |
Observed Molecular Weight | 102-105 kDa |
GenBank Accession Number | BC001626 |
Gene Symbol | MCM3 |
Gene ID (NCBI) | 4172 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P25205 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).
Protocols
Product Specific Protocols | |
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WB protocol for MCM3 antibody 84792-2-RR | Download protocol |
IF protocol for MCM3 antibody 84792-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |