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  • Western Blot (WB) analysis of various lysates using MCM3 Recombinant antibody (84792-2-RR)

    WB analysis using 84792-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84792-2-RR (MCM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using MCM3 Recombinant antibody (84792-2-RR)

    IF Staining of Raji using 84792-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using MCM3 antibody (84792-2-RR, Clone: 242103E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of K-562 cells using MCM3 Recombinant antibody (84792-2-RR)

    FC experiment of K-562 using 84792-2-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Mcm3 Recombinant Antibody (84792-2-RR, Clone:242103E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using MCM3 Recombinant antibody (84792-2-RR)

    FC experiment of HeLa using 84792-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Mcm3 Recombinant Antibody (84792-2-RR, Clone:242103E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

MCM3 Recombinant antibody

MCM3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84792-2-RR
Clone No.242103E4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inK-562 cells, Raji cells, HL-60 cells
Positive IF/ICC detected inRaji cells
Positive FC (Intra) detected inK-562 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84792-2-RR targets MCM3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MCM3 fusion protein Ag7966 Predict reactive species
Full Name minichromosome maintenance complex component 3
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 102-105 kDa
GenBank Accession NumberBC001626
Gene Symbol MCM3
Gene ID (NCBI) 4172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25205
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).

Protocols

Product Specific Protocols
WB protocol for MCM3 antibody 84792-2-RRDownload protocol
IF protocol for MCM3 antibody 84792-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols