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RNF4 Recombinant antibody

RNF4 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242197A10

Cat no : 84770-1-RR

Synonyms

RES4 26, Protein SNURF, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF4, E3 ubiquitin ligase RNF4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF4 Recombinant antibody (84770-1-RR)

    IF Staining of HepG2 using 84770-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF4 antibody (84770-1-RR, Clone: 242197A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84770-1-RR targets RNF4 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF4 fusion protein Ag12115
Full Name ring finger protein 4
Calculated Molecular Weight 190 aa, 21 kDa
GenBank Accession NumberBC031935
Gene Symbol RNF4
Gene ID (NCBI) 6047
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220].

Protocols

Product Specific Protocols
IF protocol for RNF4 antibody 84770-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols