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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNF1B Recombinant antibody (84684-5-RR)

    IF Staining of HepG2 using 84684-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using HNF1B Recombinant antibody (84684-5-RR)

    IF Staining of Caco-2 using 84684-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Caco-2 cells using HNF1B Recombinant antibody (84684-5-RR)

    FC experiment of Caco-2 using 84684-5-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug HNF1B Recombinant antibody (84684-5-RR, Clone:242120H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84684-5-RR

    Affinity and Kinetic Characterization of 84684-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84684-5-RR against Human HNF1B were performed. The affinity constant is 13.5 nM.

HNF1B Recombinant antibody

HNF1B {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84684-5-RR
Clone No.242120H8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IF/ICC detected inHepG2 cells, Caco-2 cells
Positive FC (Intra) detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84684-5-RR targets HNF1B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNF1B fusion protein Ag3232 Predict reactive species
Full Name HNF1 homeobox B
Calculated Molecular Weight 557 aa, 61 kDa
GenBank Accession NumberBC017714
Gene Symbol HNF1B
Gene ID (NCBI) 6928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP35680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis.

Protocols

Product Specific Protocols
IF protocol for HNF1B antibody 84684-5-RRDownload protocol
FC protocol for HNF1B antibody 84684-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols