Various lysates were subjected to SDS PAGE followed by western blot with 84673-5-RR (SSRP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84673-5-RR (SSRP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
FC experiment of HeLa using 84673-5-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
FC experiment of SH-SY5Y using 84673-5-RR
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Various lysates were subjected to SDS PAGE followed by western blot with 84673-5-RR (SSRP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
FC (INTRA) Figures
FC experiment of HeLa using 84673-5-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
FC experiment of SH-SY5Y using 84673-5-RR
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SSRP1 Recombinant antibody
Catalog Number
84673-5-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet