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CD31 Recombinant antibody

CD31 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242041E12

Cat no : 84607-4-RR

Synonyms

Pecam1, PECAM 1, Pecam, 242041E12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD31 Recombinant antibody (84607-4-RR)

    WB analysis using 84607-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84607-4-RR (CD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of bEnd.3 cells using CD31 Recombinant antibody (84607-4-RR)

    IF Staining of bEnd.3 using 84607-4-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31/PECAM-1 antibody (84607-4-RR, Clone: 242041E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inbEnd.3 cells, mouse lung tissue
Positive IF/ICC detected inbEnd.3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84607-4-RR targets CD31 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name platelet/endothelial cell adhesion molecule 1
Calculated Molecular Weight81 kDa
Observed Molecular Weight100-130 kDa
GenBank Accession NumberNM_001305157.1
Gene Symbol CD31
Gene ID (NCBI) 18613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
WB protocol for CD31 antibody 84607-4-RRDownload protocol
IF protocol for CD31 antibody 84607-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols