Mouse CD31 Recombinant antibody, PBS Only

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  WB analysis using 84607-4-RR (same clone as 84607-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84607-4-RR (CD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84607-4-PBS in a different storage buffer formulation.

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  IF Staining of bEnd.3 using 84607-4-RR (same clone as 84607-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31/PECAM-1 antibody (84607-4-RR, Clone: 242041E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84607-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84607-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84607-4-RR against Mouse CD31 were performed. The affinity constant is 0.882 nM.

CD31 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84607-4-PBS

Clone No.242041E12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Pecam1, PECAM 1, Pecam, 242041E12

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Product Information

84607-4-PBS targets CD31 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: platelet/endothelial cell adhesion molecule 1
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: NM_001305157.1
• Gene Symbol: CD31
• Gene ID (NCBI): 18613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q08481-1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).