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MAN1B1 Recombinant antibody

MAN1B1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241817E10

Cat no : 84588-1-RR

Synonyms

ER mannosidase 1, ER alpha-1,2-mannosidase, ER alpha 1,2 mannosidase, Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, EC:3.2.1.113

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAN1B1 Recombinant antibody (84588-1-RR)

    WB analysis using 84588-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAN1B1 Recombinant antibody (84588-1-RR)

    WB analysis using 84588-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84588-1-RR (MAN1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U2OS cells using MAN1B1 Recombinant antibody (84588-1-RR)

    FC experiment of U2OS using 84588-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.2 ug MAN1B1 Recombinant antibody (84588-1-RR, Clone:241817E10) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, HeLa cells, LNCaP cells, HEK-293 cells, U-87 MG cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84588-1-RR targets MAN1B1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAN1B1 fusion protein Ag28302
Full Name mannosidase, alpha, class 1B, member 1
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC002953
Gene Symbol MAN1B1
Gene ID (NCBI) 11253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).

Protocols

Product Specific Protocols
WB protocol for MAN1B1 antibody 84588-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols