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GINS2 Recombinant antibody

GINS2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241870E9

Cat no : 84486-2-RR

Synonyms

PSF2, Pfs2, HSPC037, GINS 2, DC5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSF2 Recombinant antibody (84486-2-RR)

    WB analysis using 84486-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84486-2-RR (GINS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSF2 Recombinant antibody (84486-2-RR)

    IF Staining of HeLa using 84486-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GINS2 antibody (84486-2-RR, Clone: 241870E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84486-2-RR

    Affinity and Kinetic Characterization of 84486-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84486-2-RR against Human GINS2 were performed. The affinity constant is 0.82 nM.

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84486-2-RR targets GINS2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GINS2 fusion protein Ag9180
Full Name GINS complex subunit 2 (Psf2 homolog)
Calculated Molecular Weight 185 aa, 21 kDa
Observed Molecular Weight20-25 kDa
GenBank Accession NumberBC010164
Gene Symbol GINS2
Gene ID (NCBI) 51659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.

Protocols

Product Specific Protocols
WB protocol for GINS2 antibody 84486-2-RRDownload protocol
IF protocol for GINS2 antibody 84486-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols