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SYNGR1 Recombinant antibody

SYNGR1 Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241460D1

Cat no : 84161-3-RR

Synonyms

Synaptogyrin-1, synaptogyrin 1, 241460D1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNGR1 Recombinant antibody (84161-3-RR)

    WB analysis using 84161-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84161-3-RR (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using SYNGR1 Recombinant antibody (84161-3-RR)

    WB analysis of pig brain using 84161-3-RR

    pig brain tissue was subjected to SDS PAGE followed by western blot with 84161-3-RR (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SYNGR1 Recombinant antibody (84161-3-RR)

    IF Staining of mouse brain using 84161-3-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNGR1 antibody (84161-3-RR, Clone: 241460D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84161-3-RR

    Affinity and Kinetic Characterization of 84161-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84161-3-RR against Human SYNGR1 were performed. The affinity constant is 3.48 nM.

Tested Applications

Positive WB detected inmouse brain tissue, pig brain tissue, rat brain tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84161-3-RR is SYNGR1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SYNGR1 fusion protein Ag0685
Full Name synaptogyrin 1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC000731
Gene Symbol SYNGR1
Gene ID (NCBI) 9145
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptogyrins are one of the most abundant vesicle components and are involved in the regulation of neurotransmitter release and synaptic plasticity. Synaptogyrins comprise a family of tyrosine-phosphorylated proteins with three isoforms: two neuronal forms (synaptogyrin-1 and -3) and one ubiquitous form (synaptogyrin-2). The synaptogyrin 1 (SYNGR1) can act as a regulator of Ca2+-dependent exocytosis. SYNGR1 protein mutations are associated with schizophrenia and are considered to be positional candidate genes for schizophrenia.(PMID: 10383386; 10595519; 14755516; 14732601; 17049558)

Protocols

Product Specific Protocols
WB protocol for SYNGR1 antibody 84161-3-RRDownload protocol
IF protocol for SYNGR1 antibody 84161-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols