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MERTK Recombinant antibody

MERTK Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241252H3

Cat no : 84156-2-RR

Synonyms

Proto-oncogene c-Mer, Proto oncogene c Mer, MER, EC:2.7.10.1, c mer

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MERTK Recombinant antibody (84156-2-RR)

    WB analysis using 84156-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84156-2-RR (MERTK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MERTK Recombinant antibody (84156-2-RR)

    IHC staining of mouse kidney using 84156-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84156-2-RR (MERTK antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84156-2-RR

    Affinity and Kinetic Characterization of 84156-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84156-2-RR against Human MERTK were performed. The affinity constant is 0.444 nM.

Tested Applications

Positive WB detected inU-937 cells, HEK-293T cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84156-2-RR is MERTK Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MERTK fusion protein Ag27540
Full Name c-mer proto-oncogene tyrosine kinase
Calculated Molecular Weight 999 aa, 110 kDa
Observed Molecular Weight160-180 kDa
GenBank Accession NumberBC114918
Gene Symbol MERTK
Gene ID (NCBI) 10461
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MerTK (Mer tyrosine kinase), also known as RP38, c-Eyk, c-mer, and Tyro12, was first cloned from a human B lymphoblastoid expression library (PMID:8086340) and is one of the TAM (Tyro-3, Axl, and MerTK) receptor tyrosine kinase (RTK) family (PMID:23833304). Although this RTK, like others, can promote tumor cell proliferation to some extent, MERTK primarily lends tumor cells crucial survival advantages while promoting invasion, migration and metastasis, drug resistance, and, in the innate immune system, suppressing anti-tumor immunity (PMID:32417270). The multiple MERTK species observed are likely due to posttranslational modifications (PMID: 23585477).

Protocols

Product Specific Protocols
WB protocol for MERTK antibody 84156-2-RRDownload protocol
IHC protocol for MERTK antibody 84156-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols