Filter: AllWBSandwich ELISASample testIF/ICCFC (Intra)Cytometric bead arrayAffinity

WB analysis using 84131-2-RR (same clone as 84131-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84131-2-RR (EIF3F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP01038-4
Sandwich ELISA standard curve of MP01038-4, Human EIF3F Recombinant Matched Antibody Pair - PBS only. 84131-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34849. 84131-2-PBS was HRP conjugated as the detection antibody. Range: 0.469-30 ng/mL.
Sample test of MP01038-4
The mean EIF3F concentration was determined to be 225.37 ng/mL in Jurkat cell extract based on a 1.60 mg/mL extract load.
IF Staining of HeLa using 84131-2-RR (same clone as 84131-2-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF3F antibody (84131-2-RR, Clone: 240894D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.
FC experiment of HeLa using 84131-2-RR (same clone as 84131-2-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug EIF3F Recombinant antibody (84131-2-RR, Clone:240894D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.
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1x10^6 HeLa cells were intracellularly stained with 0.25 ug EIF3F Recombinant antibody (84131-2-RR, Clone:240894D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP01038-1
Cytometric bead array standard curve of MP01038-1, EIF3F Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84131-2-PBS. Detection antibody: 84131-1-PBS. Standard: Ag34849. Range: 0.625-80 ng/mL.
Cytometric bead array standard curve of MP01038-2
Cytometric bead array standard curve of MP01038-2, EIF3F Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84131-2-PBS. Detection antibody: 84131-3-PBS. Standard: Ag34849. Range: 0.625-80 ng/mL.
Affinity and Kinetic Characterization of 84131-2-RR
Biolayer interferometry (BLl) kinetic assays of 84131-2-RR against Human EIF3F were performed. The affinity constant is 1.78 nM.

EIF3F Recombinant antibody, PBS Only (Capture/Detector)

EIF3F {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84131-2-PBS

Clone No.240894D2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

eIF3 p47, 240894D2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84131-2-PBS targets EIF3F as part of a matched antibody pair:

MP01038-1: 84131-2-PBS capture and 84131-1-PBS detection (validated in Cytometric bead array)

MP01038-2: 84131-2-PBS capture and 84131-3-PBS detection (validated in Cytometric bead array)

MP01038-4: 84131-1-PBS capture and 84131-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	EIF3F fusion protein Ag34849 Predict reactive species
Full Name	eukaryotic translation initiation factor 3, subunit F
Calculated Molecular Weight	38 kDa
Observed Molecular Weight	40-45 kDa
GenBank Accession Number	BC000490
Gene Symbol	EIF3F
Gene ID (NCBI)	8665
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O00303
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

EIF3F (eukaryotic translation initiation factor 3 subunit F) is a protein that plays an important role in eukaryotic cells and is an integral part of the translation initiation factor 3(Eif3) complex. EIF3 is a complex of at least 10 distinct subunits and is the largest translation initiation factor in eukaryotes.