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  • Western Blot (WB) analysis of various lysates using EIF3F Recombinant antibody (84131-2-RR)

    WB analysis using 84131-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84131-2-RR (EIF3F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF3F Recombinant antibody (84131-2-RR)

    IF Staining of HeLa using 84131-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF3F antibody (84131-2-RR, Clone: 240894D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using EIF3F Recombinant antibody (84131-2-RR)

    FC experiment of HeLa using 84131-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug EIF3F Recombinant antibody (84131-2-RR, Clone:240894D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84131-2-RR

    Affinity and Kinetic Characterization of 84131-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84131-2-RR against Human EIF3F were performed. The affinity constant is 1.78 nM.

EIF3F Recombinant antibody

EIF3F {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84131-2-RR
Clone No.240894D2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

eIF3 p47, 240894D2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inU2OS cells, HeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84131-2-RR targets EIF3F in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EIF3F fusion protein Ag34849 Predict reactive species
Full Name eukaryotic translation initiation factor 3, subunit F
Calculated Molecular Weight 38 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC000490
Gene Symbol EIF3F
Gene ID (NCBI) 8665
RRIDAB_3671689
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00303
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF3F (eukaryotic translation initiation factor 3 subunit F) is a protein that plays an important role in eukaryotic cells and is an integral part of the translation initiation factor 3(Eif3) complex. EIF3 is a complex of at least 10 distinct subunits and is the largest translation initiation factor in eukaryotes.

Protocols

Product Specific Protocols
WB protocol for EIF3F antibody 84131-2-RRDownload protocol
IF protocol for EIF3F antibody 84131-2-RRDownload protocol
FC protocol for EIF3F antibody 84131-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols