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BCRP/ABCG2 Recombinant antibody, PBS Only (Capture)

BCRP/ABCG2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240959A4

Cat no : 83835-1-PBS

Synonyms

ABCG2, BCRP,ABCG2, BCRP, ABCP, ABC15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCRP/ABCG2 Recombinant antibody (83835-1-RR)

    WB analysis using 83835-1-RR (same clone as 83835-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83835-1-RR (BCRP,ABCG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83835-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00805-1

    Cytometric bead array standard curve of MP00805-1

    Cytometric bead array standard curve of MP00805-1, BCRP/ABCG2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83835-1-PBS. Detection antibody: 83835-2-PBS. Standard: Ag25911. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83835-1-RR

    Affinity and Kinetic Characterization of 83835-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83835-1-RR against Human BCRP,ABCG2 were performed. The affinity constant is 9.85 nM.

Product Information

83835-1-PBS targets BCRP/ABCG2 as part of a matched antibody pair:

MP00805-1: 83835-1-PBS capture and 83835-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCRP/ABCG2 fusion protein Ag25911
Full Name ATP-binding cassette, sub-family G (WHITE), member 2
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC021281
Gene Symbol ABCG2
Gene ID (NCBI) 9429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.