BCRP,ABCG2 Recombinant antibody

BCRP,ABCG2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240959A4

Cat no : 83835-1-RR

Synonyms

ABCG2, BCRP/ABCG2, BCRP, ABCP, ABC15

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Validation Data Gallery

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  WB analysis using 83835-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83835-1-RR (BCRP,ABCG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83835-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83835-1-RR against Human BCRP,ABCG2 were performed. The affinity constant is 9.85 nM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, U-251 cells, MCF-7 cells, SGC-7901 cells, HepG2 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83835-1-RR is BCRP,ABCG2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCRP,ABCG2 fusion protein Ag25911
• Full Name: ATP-binding cassette, sub-family G (WHITE), member 2
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: BC021281
• Gene Symbol: ABCG2
• Gene ID (NCBI): 9429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.