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  • Western Blot (WB) analysis of various lysates using GATA3 Recombinant antibody (83808-1-RR)

    WB analysis using 83808-1-RR (same clone as 83808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using GATA3 Recombinant antibody (83808-1-RR)

    WB analysis of MCF-7 using 83808-1-RR (same clone as 83808-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using GATA3 Recombinant antibody (83808-1-RR)

    FC experiment of Jurkat using 83808-1-RR (same clone as 83808-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U937 using GATA3 Recombinant antibody (83808-1-RR)

    FC experiment of U937 using 83808-1-RR (same clone as 83808-1-PBS)

    1x10^6 U937 were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01933-1

    Cytometric bead array standard curve of MP01933-1

    Cytometric bead array standard curve of MP01933-1, GATA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83808-1-PBS. Detection antibody: 83808-4-PBS. Standard: Ag0174. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83808-1-RR

    Affinity and Kinetic Characterization of 83808-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83808-1-RR against Human GATA3 were performed. The affinity constant is 0.686 nM.

GATA3 Recombinant antibody, PBS Only (Capture)

GATA3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83808-1-PBS
Clone No.240684A2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684A2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

83808-1-PBS targets GATA3 as part of a matched antibody pair:

MP01933-1: 83808-1-PBS capture and 83808-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GATA3 fusion protein Ag0174 Predict reactive species
Full Name GATA binding protein 3
Calculated Molecular Weight 443 aa, 48 kDa
Observed Molecular Weight 37 kDa and 48 kDa
GenBank Accession NumberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23771
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Trans-acting T-cell-specific transcription factor, also known as GATA3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin, and mammary gland (PMID: 25148023). GATA3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full-length GATA3 protein contains 444 aa corresponding to the molecular weights of 48 kDa, and the truncated protein was detected at approximately 37 kDa (PMID: 24758297).