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GATA3 Recombinant antibody

GATA3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240684A2

Cat no : 83808-1-RR

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684A2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GATA3 Recombinant antibody (83808-1-RR)

    WB analysis using 83808-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GATA3 Recombinant antibody (83808-1-RR)

    WB analysis of MCF-7 using 83808-1-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using GATA3 Recombinant antibody (83808-1-RR)

    FC experiment of Jurkat using 83808-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of U937 using GATA3 Recombinant antibody (83808-1-RR)

    FC experiment of U937 using 83808-1-RR

    1x10^6 U937 were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83808-1-RR

    Affinity and Kinetic Characterization of 83808-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83808-1-RR against Human GATA3 were performed. The affinity constant is 0.686 nM.

Tested Applications

Positive WB detected inEL-4 cells, MCF-7 cells, SH-SY5Y cells, T-47D cells
Positive FC (Intra) detected inJurkat cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83808-1-RR is GATA3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GATA3 fusion protein Ag0174
Full Name GATA binding protein 3
Calculated Molecular Weight 443 aa, 48 kDa
Observed Molecular Weight 37 kDa and 48 kDa
GenBank Accession NumberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Trans-acting T-cell-specific transcription factor, also known as GATA3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin, and mammary gland (PMID: 25148023). GATA3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full-length GATA3 protein contains 444 aa corresponding to the molecular weights of 48 kDa, and the truncated protein was detected at approximately 37 kDa (PMID: 24758297).

Protocols

Product Specific Protocols
WB protocol for GATA3 antibody 83808-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols