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EGFR Recombinant antibody

EGFR Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240603F10

Cat no : 83806-1-RR

Synonyms

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 240603F10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EGFR Recombinant antibody (83806-1-RR)

    WB analysis using 83806-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83806-1-RR (EGFR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EGFR Recombinant antibody (83806-1-RR)

    IHC staining of human placenta using 83806-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using EGFR Recombinant antibody (83806-1-RR)

    IHC staining of human placenta using 83806-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EGFR Recombinant antibody (83806-1-RR)

    IF Staining of A431 using 83806-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EGFR Recombinant antibody (83806-1-RR)

    IF Staining of A431 using 83806-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using EGFR Recombinant antibody (83806-1-RR)

    FC experiment of A431 using 83806-1-RR

    1x10^6 A431 cells (left) or Jurkat cells (right) were intracellularly stained with 0.4 ug EGFR Recombinant antibody (83806-1-RR, Clone: 240603F10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA .

Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83806-1-RR targets EGFR in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated Molecular Weight 134 kDa
Observed Molecular Weight145-165 kDa
GenBank Accession NumberNM_005228
Gene Symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

Protocols

Product Specific Protocols
WB protocol for EGFR antibody 83806-1-RRDownload protocol
IHC protocol for EGFR antibody 83806-1-RRDownload protocol
IF protocol for EGFR antibody 83806-1-RRDownload protocol
FC protocol for EGFR antibody 83806-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols