EGFR Recombinant antibody, PBS Only

EGFR Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240603F10

Cat no : 83806-1-PBS

Synonyms

EGFR-Specific, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R

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Validation Data Gallery

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  WB analysis using 83806-1-RR (same clone as 83806-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83806-1-RR (EGFR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83806-1-RR (same clone as 83806-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83806-1-RR (same clone as 83806-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83806-1-RR (same clone as 83806-1-PBS)

  1x10^6 A431 cells (left) or Jurkat cells (right) were intracellularly stained with 0.4 ug EGFR Recombinant antibody (83806-1-RR, Clone: 240603F10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

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Product Information

83806-1-PBS targets EGFR in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 145-165 kDa
• GenBank Accession Number: NM_005228
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.