EGFR Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 83806-1-RR (same clone as 83806-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83806-1-RR (EGFR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 83806-1-RR (same clone as 83806-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 83806-1-RR (same clone as 83806-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HaCaT using 83806-1-RR (same clone as 83806-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using EGFR antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83806-1-RR (same clone as 83806-1-PBS)

  1x10^6 A431 cells (left) or Jurkat cells (right) were intracellularly stained with 0.4 ug EGFR Recombinant antibody (83806-1-RR, Clone: 240603F10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

EGFR Uni-rAb^® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Cat No. 83806-1-PBS

Clone No.240603F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Synonyms

EGFR-Specific, 240603F10, EGF R, EGF Receptor , Epidermal growth factor receptor

---

Product Information

83806-1-PBS targets EGFR in WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 145-165 kDa
• GenBank Accession Number: NM_005228
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P00533
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.