Filter: AllWBIHCFC (Intra)Affinity

WB analysis using 83646-6-RR
Various lysates were subjected to SDS PAGE followed by western blot with 83646-6-RR (SWAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of mouse spleen using 83646-6-RR
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 83646-6-RR
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of SK-N-SH using 83646-6-RR
1x10^6 SK-N-SH cells were intracellularly stained with 0.25 ug SWAP70 Recombinant antibody (83646-6-RR, Clone:240641F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of U2OS using 83646-6-RR
1x10^6 U2OS cells were intracellularly stained with 0.2 ug SWAP70 Recombinant antibody (83646-6-RR, Clone:240641F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 83646-6-RR
Biolayer interferometry (BLl) kinetic assays of 83646-6-RR against Human SWAP70 were performed. The affinity constant is below 1 pM.

SWAP70 Recombinant antibody

SWAP70 {ptg:Brand} Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83646-6-RR

Clone No.240641F5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

SWAP-70, SWAP 70 protein, SWAP 70, KIAA0640, HSPC321

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, NIH/3T3 cells, Daudi cells, NIC-H1299 cells, HEK-293 cells, A549 cellls
Positive IHC detected in	mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	SK-N-SH cells, U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83646-6-RR targets SWAP70 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	SWAP70 fusion protein Ag34855 Predict reactive species
Full Name	SWAP-70 protein
Calculated Molecular Weight	69 kDa
Observed Molecular Weight	65-70 kDa
GenBank Accession Number	BC000616
Gene Symbol	SWAP70
Gene ID (NCBI)	23075
RRID	AB_3671255
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q9UH65
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SWAP70 (switching B cell complex subunit), also known as HSPC321. It is expected to be located in cell membrane, cytoplasm and nucleus, the protein is mainly expressed in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes, macrophages and mature B-cells (PMID: 10681448). The calculated molecular weight of SWAP70 is 69 kDa. SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. It is reported that the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. It is suggested that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation (PMID: 10681448).