SWAP70 Recombinant antibody, PBS Only

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  WB analysis using 83646-6-RR (same clone as 83646-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83646-6-RR (SWAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  FC experiment of SK-N-SH using 83646-6-RR (same clone as 83646-6-PBS)

  1x10^6 SK-N-SH cells were intracellularly stained with 0.25 ug SWAP70 Recombinant antibody (83646-6-RR, Clone:240641F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83646-6-RR (same clone as 83646-6-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.2 ug SWAP70 Recombinant antibody (83646-6-RR, Clone:240641F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83646-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83646-6-RR against Human SWAP70 were performed. The affinity constant is below 1 pM.

SWAP70 {ptg:Brand} Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83646-6-PBS

Clone No.240641F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

SWAP-70, SWAP 70 protein, SWAP 70, KIAA0640, HSPC321

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Product Information

83646-6-PBS targets SWAP70 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SWAP70 fusion protein Ag34855
• Full Name: SWAP-70 protein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC000616
• Gene Symbol: SWAP70
• Gene ID (NCBI): 23075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UH65
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SWAP70 (switching B cell complex subunit), also known as HSPC321. It is expected to be located in cell membrane, cytoplasm and nucleus, the protein is mainly expressed in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes, macrophages and mature B-cells (PMID: 10681448). The calculated molecular weight of SWAP70 is 69 kDa. SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. It is reported that the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. It is suggested that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation (PMID: 10681448).