TMEM53 Recombinant antibody, PBS Only

TMEM53 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240511C3

Cat no : 83554-3-PBS

Synonyms

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

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Validation Data Gallery

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  IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 83554-3-RR (same clone as 83554-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug TMEM53 Recombinant antibody (83554-3-RR, Clone:240511C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83554-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83554-3-RR against Human TMEM53 were performed. The affinity constant is 44.5 nM.

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Product Information

The immunogen of 83554-3-PBS is TMEM53 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM53 fusion protein Ag21321
• Full Name: transmembrane protein 53
• Calculated Molecular Weight: 277 aa, 32 kDa
• GenBank Accession Number: BC064520
• Gene Symbol: TMEM53
• Gene ID (NCBI): 79639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.