Filter: AllWBFC (Intra)Cytometric bead arrayAffinity

WB analysis of HepG2 using 83510-1-RR (same clone as 83510-1-PBS)
WB result of MED21 antibody (83510-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED21 transfected HepG2 cells. This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.
WB analysis using 83510-1-RR (same clone as 83510-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83510-1-RR (MED21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 83510-1-RR (same clone as 83510-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.
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1x10^6 HeLa cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.
FC experiment of U-87 MG using 83510-1-RR (same clone as 83510-1-PBS)
1x10^6 U-87 MG cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.
×
1x10^6 U-87 MG cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP00506-1
Cytometric bead array standard curve of MP00506-1, MED21 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83510-1-PBS. Detection antibody: 83510-2-PBS. Standard: Ag8568. Range: 1.25-40 ng/mL.
Affinity and Kinetic Characterization of 83510-1-RR
Biolayer interferometry (BLl) kinetic assays of 83510-1-RR against Human MED21 were performed. The affinity constant is 2.06 nM.

Featured Product
KD/KO Validated

MED21 Recombinant antibody, PBS Only (Capture)

MED21 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83510-1-PBS

Clone No.240419E2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

SURB7, SRB7, hSrb7, 240419E2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

83510-1-PBS targets MED21 as part of a matched antibody pair:

MP00506-1: 83510-1-PBS capture and 83510-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	MED21 fusion protein Ag8568 Predict reactive species
Full Name	mediator complex subunit 21
Calculated Molecular Weight	144 aa, 16 kDa
Observed Molecular Weight	13-16 kDa
GenBank Accession Number	BC008380
Gene Symbol	MED21
Gene ID (NCBI)	9412
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13503
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

MED21, also known as SRB7 and SURB7,belongs to the Mediator complex subunit 21 family. MED21 is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. MED21 is thought to play an important role in transcriptional activation and repression. MED21 regulates the transcription of nearly all RNA polymerase II-dependent genes (PMID: 16758199, PMID: 30559736).