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  • Western Blot (WB) analysis of human testis tissue using MDC1 Recombinant antibody (83496-1-RR)

    WB analysis of human testis using 83496-1-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MDC1 Recombinant antibody (83496-1-RR)

    WB analysis using 83496-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MDC1 Recombinant antibody (83496-1-RR)

    IF Staining of HeLa using 83496-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MDC1 antibody (83496-1-RR, Clone: 240469D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83496-1-RR

    Affinity and Kinetic Characterization of 83496-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83496-1-RR against Human MDC1 were performed. The affinity constant is 0.346 nM.

MDC1 Recombinant antibody

MDC1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83496-1-RR
Clone No.240469D5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NFBD1, NFBD 1, MDC 1, 240469D5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman testis tissue, HeLa cells, HEK-293 cells, MCF-7 cells, K-562 cells, PC-3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83496-1-RR targets MDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MDC1 fusion protein Ag20135 Predict reactive species
Full Name mediator of DNA damage checkpoint 1
Calculated Molecular Weight 2089 aa, 227 kDa
Observed Molecular Weight260-320 kDa
GenBank Accession NumberBC152556
Gene Symbol MDC1
Gene ID (NCBI) 9656
RRIDAB_3671123
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14676
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

Protocols

Product Specific Protocols
WB protocol for MDC1 antibody 83496-1-RRDownload protocol
IF protocol for MDC1 antibody 83496-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols