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FAM38B Recombinant antibody, PBS Only (Capture)

FAM38B Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240406E5

Cat no : 83488-4-PBS

Synonyms

Piezo2, Transmembrane protein C18orf30, C18orf30, 240406E5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FAM38B Recombinant antibody (83488-4-RR)

    WB analysis of HeLa using 83488-4-RR (same clone as 83488-4-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 83488-4-RR (FAM38B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83488-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using FAM38B Recombinant antibody (83488-4-RR)

    FC experiment of A431 using 83488-4-RR (same clone as 83488-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Fam38b Recombinant Antibody (83488-4-RR, Clone:240406E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83488-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00488-2

    Cytometric bead array standard curve of MP00488-2

    Cytometric bead array standard curve of MP00488-2, FAM38B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83488-4-PBS. Detection antibody: 83488-1-PBS. Standard: Ag24528. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00488-3

    Cytometric bead array standard curve of MP00488-3

    Cytometric bead array standard curve of MP00488-3, FAM38B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83488-4-PBS. Detection antibody: 83488-2-PBS. Standard: Ag24528. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83488-4-RR

    Affinity and Kinetic Characterization of 83488-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83488-4-RR against Human FAM38B were performed. The affinity constant is 3.8 nM.

Product Information

83488-4-PBS targets FAM38B as part of a matched antibody pair:

MP00488-2: 83488-4-PBS capture and 83488-1-PBS detection (validated in Cytometric bead array)

MP00488-3: 83488-4-PBS capture and 83488-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FAM38B fusion protein Ag24528
Full Name family with sequence similarity 38, member B
Calculated Molecular Weight 318 kDa
Observed Molecular Weight250-310 kDa, 80 kDa
GenBank Accession NumberAB527139
Gene Symbol FAM38B
Gene ID (NCBI) 63895
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FAM38B, also named as PIEZO2, is a mechanosensitive, rapidly inactivating (RI) ion channel which is open and converts the mechanical stimulus signals into bioelectrical signals after stimulated by mechanical signals. FAM38B has been recently identified in dorsal root ganglion (DRG) neurons to mediate tactile transduction. It plays an important role in the biological process, maintaining cell metabolism and cell migration. Loss-of-function mutations in the human FAM38B gene cause an autosomal recessive syndrome of muscular atrophy with perinatal respiratory distress, arthrogryposis, and scoliosis.The 80 kDa band detected by SDS-PAGE can be caused by alternative splicing (PMID: 34335288, 37227654).