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FAM38B Recombinant antibody

FAM38B Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240406E5

Cat no : 83488-4-RR

Synonyms

Piezo2, Transmembrane protein C18orf30, C18orf30, 240406E5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FAM38B Recombinant antibody (83488-4-RR)

    WB analysis of HeLa using 83488-4-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 83488-4-RR (FAM38B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using FAM38B Recombinant antibody (83488-4-RR)

    FC experiment of A431 using 83488-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Fam38b Recombinant Antibody (83488-4-RR, Clone:240406E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83488-4-RR

    Affinity and Kinetic Characterization of 83488-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83488-4-RR against Human FAM38B were performed. The affinity constant is 3.8 nM.

Tested Applications

Positive WB detected inHeLa cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83488-4-RR is FAM38B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FAM38B fusion protein Ag24528
Full Name family with sequence similarity 38, member B
Calculated Molecular Weight 318 kDa
Observed Molecular Weight250-310 kDa, 80 kDa
GenBank Accession NumberAB527139
Gene Symbol FAM38B
Gene ID (NCBI) 63895
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM38B, also named as PIEZO2, is a mechanosensitive, rapidly inactivating (RI) ion channel which is open and converts the mechanical stimulus signals into bioelectrical signals after stimulated by mechanical signals. FAM38B has been recently identified in dorsal root ganglion (DRG) neurons to mediate tactile transduction. It plays an important role in the biological process, maintaining cell metabolism and cell migration. Loss-of-function mutations in the human FAM38B gene cause an autosomal recessive syndrome of muscular atrophy with perinatal respiratory distress, arthrogryposis, and scoliosis.The 80 kDa band detected by SDS-PAGE can be caused by alternative splicing (PMID: 34335288, 37227654).

Protocols

Product Specific Protocols
WB protocol for FAM38B antibody 83488-4-RRDownload protocol
FC protocol for FAM38B antibody 83488-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols