FAM38B Recombinant antibody

FAM38B Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240406E5

Cat no : 83488-4-RR

Synonyms

Piezo2, Transmembrane protein C18orf30, C18orf30, 240406E5

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Validation Data Gallery

• 

  WB analysis of HeLa using 83488-4-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 83488-4-RR (FAM38B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 83488-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Fam38b Recombinant Antibody (83488-4-RR, Clone:240406E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83488-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83488-4-RR against Human FAM38B were performed. The affinity constant is 3.8 nM.

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83488-4-RR is FAM38B Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FAM38B fusion protein Ag24528
• Full Name: family with sequence similarity 38, member B
• Calculated Molecular Weight: 318 kDa
• Observed Molecular Weight: 250-310 kDa, 80 kDa
• GenBank Accession Number: AB527139
• Gene Symbol: FAM38B
• Gene ID (NCBI): 63895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM38B, also named as PIEZO2, is a mechanosensitive, rapidly inactivating (RI) ion channel which is open and converts the mechanical stimulus signals into bioelectrical signals after stimulated by mechanical signals. FAM38B has been recently identified in dorsal root ganglion (DRG) neurons to mediate tactile transduction. It plays an important role in the biological process, maintaining cell metabolism and cell migration. Loss-of-function mutations in the human FAM38B gene cause an autosomal recessive syndrome of muscular atrophy with perinatal respiratory distress, arthrogryposis, and scoliosis.The 80 kDa band detected by SDS-PAGE can be caused by alternative splicing (PMID: 34335288, 37227654).