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ELAC2 Recombinant antibody

ELAC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240487B6

Cat no : 83468-6-RR

Synonyms

FLJ10530, ELC2, ElaC homolog protein 2, elaC homolog 2 (E. coli), EC:3.1.26.11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ELAC2 Recombinant antibody (83468-6-RR)

    WB analysis using 83468-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83468-6-RR (ELAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using ELAC2 Recombinant antibody (83468-6-RR)

    FC experiment of A431 using 83468-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ELAC2 Recombinant antibody (83468-6-RR, Clone:240487B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83468-6-RR

    Affinity and Kinetic Characterization of 83468-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83468-6-RR against Human ELAC2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Raji cells, DU 145 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83468-6-RR is ELAC2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ELAC2 fusion protein Ag0105
Full Name elaC homolog 2 (E. coli)
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC004158
Gene Symbol ELAC2
Gene ID (NCBI) 60528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

Protocols

Product Specific Protocols
WB protocol for ELAC2 antibody 83468-6-RRDownload protocol
FC protocol for ELAC2 antibody 83468-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols