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TBX20 Recombinant antibody, PBS Only (Capture)

TBX20 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240385H6

Cat no : 83414-3-PBS

Synonyms

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse heart tissue using TBX20 Recombinant antibody (83414-3-RR)

    IHC staining of mouse heart using 83414-3-RR (same clone as 83414-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83414-3-RR (TBX20 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83414-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using TBX20 Recombinant antibody (83414-3-RR)

    IF Staining of H9C2 using 83414-3-RR (same clone as 83414-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using TBX20 antibody (83414-3-RR, Clone: 240385H6 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83414-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K562 using TBX20 Recombinant antibody (83414-3-RR)

    FC experiment of K562 using 83414-3-RR (same clone as 83414-3-PBS)

    1x10^6 K562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-3-RR, Clone:240385H6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83414-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00401-1

    Cytometric bead array standard curve of MP00401-1

    Cytometric bead array standard curve of MP00401-1, TBX20 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83414-3-PBS. Detection antibody: 83414-1-PBS. Standard: Ag18501. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83414-3-RR

    Affinity and Kinetic Characterization of 83414-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83414-3-RR against Human TBX20 were performed. The affinity constant is 17 nM.

Product Information

83414-3-PBS targets TBX20 as part of a matched antibody pair:

MP00401-1: 83414-3-PBS capture and 83414-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TBX20 fusion protein Ag18501
Full Name T-box 20
Calculated Molecular Weight 297 aa, 33 kDa
GenBank Accession NumberBC120946
Gene Symbol TBX20
Gene ID (NCBI) 57057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.