TBX20 Recombinant antibody

TBX20 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240385H6

Cat no : 83414-3-RR

Synonyms

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

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Validation Data Gallery

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  IHC staining of mouse heart using 83414-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83414-3-RR (TBX20 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of H9C2 using 83414-3-RR

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using TBX20 antibody (83414-3-RR, Clone: 240385H6 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of K562 using 83414-3-RR

  1x10^6 K562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-3-RR, Clone:240385H6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83414-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83414-3-RR against Human TBX20 were performed. The affinity constant is 17 nM.

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Tested Applications

• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: H9C2 cells
• Positive FC (Intra) detected in: K562

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83414-3-RR is TBX20 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TBX20 fusion protein Ag18501
• Full Name: T-box 20
• Calculated Molecular Weight: 297 aa, 33 kDa
• GenBank Accession Number: BC120946
• Gene Symbol: TBX20
• Gene ID (NCBI): 57057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.