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NCAM1/CD56 Recombinant antibody

NCAM1/CD56 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240078C9

Cat no : 83365-6-RR

Synonyms

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NCAM1/CD56 Recombinant antibody (83365-6-RR)

    WB analysis of SH-SY5Y using 83365-6-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 83365-6-RR (NCAM1/CD56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using NCAM1/CD56 Recombinant antibody (83365-6-RR)

    IF Staining of SH-SY5Y using 83365-6-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NCAM1/CD56 antibody (83365-6-RR, Clone: 240078C9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using NCAM1/CD56 Recombinant antibody (83365-6-RR)

    IF Staining of SH-SY5Y using 83365-6-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NCAM1/CD56 antibody (83365-6-RR, Clone: 240078C9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity experiments of 83365-6-RR

    Affinity experiments of 83365-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83365-6-RR against Human NCAM1/CD56 were performed. The affinity constant is 3.45 nM.

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83365-6-RR is NCAM1/CD56 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name neural cell adhesion molecule 1
Calculated Molecular Weight 95 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberBC047244
Gene Symbol NCAM1
Gene ID (NCBI) 4684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

Protocols

Product Specific Protocols
WB protocol for NCAM1/CD56 antibody 83365-6-RRDownload protocol
IF protocol for NCAM1/CD56 antibody 83365-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols