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NCAM1/CD56 Recombinant antibody, PBS Only

NCAM1/CD56 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240078C9

Cat no : 83365-6-PBS

Synonyms

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NCAM1/CD56 Recombinant antibody (83365-6-RR)

    WB analysis of SH-SY5Y using 83365-6-RR (same clone as 83365-6-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 83365-6-RR (NCAM1/CD56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83365-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using NCAM1/CD56 Recombinant antibody (83365-6-RR)

    IF Staining of SH-SY5Y using 83365-6-RR (same clone as 83365-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NCAM1/CD56 antibody (83365-6-RR, Clone: 240078C9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83365-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using NCAM1/CD56 Recombinant antibody (83365-6-RR)

    IF Staining of SH-SY5Y using 83365-6-RR (same clone as 83365-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NCAM1/CD56 antibody (83365-6-RR, Clone: 240078C9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83365-6-PBS in a different storage buffer formulation.

  • Affinity experiments of 83365-6-RR

    Affinity experiments of 83365-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83365-6-RR against Human NCAM1/CD56 were performed. The affinity constant is 3.45 nM.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83365-6-PBS is NCAM1/CD56 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name neural cell adhesion molecule 1
Calculated Molecular Weight 95 kDa
GenBank Accession NumberBC047244
Gene Symbol NCAM1
Gene ID (NCBI) 4684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).