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Mouse HO-1/Hmox1 Recombinant antibody, PBS Only (Capture)

HO-1/Hmox1 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240236G4

Cat no : 83291-3-PBS

Synonyms

Hmox1, HO-1, Hmox, Hemox, heme oxygenase (decycling) 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HO-1/Hmox1 Recombinant antibody (83291-3-RR)

    WB analysis using 83291-3-RR (same clone as 83291-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using HO-1/Hmox1 Recombinant antibody (83291-3-RR)

    IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using HO-1/Hmox1 Recombinant antibody (83291-3-RR)

    IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using HO-1/Hmox1 Recombinant antibody (83291-3-RR)

    FC experiment of NIH/3T3 using 83291-3-RR (same clone as 83291-3-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug HO-1/Hmox1 Recombinant antibody (83291-3-RR, Clone:240236G4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00318-2

    Cytometric bead array standard curve of MP00318-2

    Cytometric bead array standard curve of MP00318-2, MOUSE HO-1/Hmox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83291-3-PBS. Detection antibody: 83291-2-PBS. Standard: Eg0938. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83291-3-RR

    Affinity and Kinetic Characterization of 83291-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83291-3-RR against Mouse HO-1/Hmox1 were performed. The affinity constant is 1.23 nM.

Product Information

83291-3-PBS targets HO-1/Hmox1 as part of a matched antibody pair:

MP00318-2: 83291-3-PBS capture and 83291-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name heme oxygenase (decycling) 1
Calculated Molecular Weight33 kDa
GenBank Accession NumberNM_010442.2
Gene Symbol HO-1
Gene ID (NCBI) 15368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.