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HO-1/Hmox1 Recombinant antibody

HO-1/Hmox1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240236G4

Cat no : 83291-3-RR

Synonyms

Hmox1, HO-1, Hmox, Hemox, heme oxygenase (decycling) 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HO-1/Hmox1 Recombinant antibody (83291-3-RR)

    WB analysis using 83291-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using HO-1/Hmox1 Recombinant antibody (83291-3-RR)

    IHC staining of mouse spleen using 83291-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using HO-1/Hmox1 Recombinant antibody (83291-3-RR)

    IHC staining of mouse spleen using 83291-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of NIH/3T3 cells using HO-1/Hmox1 Recombinant antibody (83291-3-RR)

    FC experiment of NIH/3T3 using 83291-3-RR

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug HO-1/Hmox1 Recombinant antibody (83291-3-RR, Clone:240236G4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83291-3-RR

    Affinity and Kinetic Characterization of 83291-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83291-3-RR against Mouse HO-1/Hmox1 were performed. The affinity constant is 1.23 nM.

Tested Applications

Positive WB detected inC2C12 cells, NIH/3T3 cells, RAW 264.7 cells, 4T1 cells, mouse liver tissue, mouse kidney tissue, mouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83291-3-RR is HO-1/Hmox1 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name heme oxygenase (decycling) 1
Calculated Molecular Weight33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberNM_010442.2
Gene Symbol HO-1
Gene ID (NCBI) 15368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HO-1/Hmox1 antibody 83291-3-RRDownload protocol
IHC protocol for HO-1/Hmox1 antibody 83291-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols