Sandwich ELISA standard curve of MP00318-3, mouse HO-1/Hmox Recombinant Matched Antibody Pair - PBS only. 83291-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0938. 83291-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

Mouse spleen tissue (3 spleen in 1-2 mm pieces in 100 mL of medium) was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse HO-1/Hmox1,and measured 225.2 pg/mL.

The mean mouse HO-1/Hmox1 concentration was determined to be 23,262.0 pg/mL in NIH/3T3 cell extract based on a 7.8 mg/mL extract load and 41,977.1 pg/mL in mouse liver extract based on a 5 mg/mL extract load.

Serum of sixteen mice was measured. The mouse HO-1/Hmox1 concentration of detected samples was determined to be 547.1 pg/mL with a range of 297.1 - 1,158.6 pg/mL.

Cytometric bead array standard curve of MP00318-1, MOUSE HO-1/Hmox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83291-1-PBS. Detection antibody: 83291-2-PBS. Standard: Eg0938. Range: 1.25-20 ng/mL.

Cytometric bead array standard curve of MP00318-2, MOUSE HO-1/Hmox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83291-3-PBS. Detection antibody: 83291-2-PBS. Standard: Eg0938. Range: 1.25-20 ng/mL.