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DOCK8 Recombinant antibody

DOCK8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230441A2

Cat no : 83270-2-RR

Synonyms

MRD2, dedicator of cytokinesis 8, 230441A2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOCK8 Recombinant antibody (83270-2-RR)

    WB analysis using 83270-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83270-2-RR (DOCK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DOCK8 Recombinant antibody (83270-2-RR)

    IHC staining of mouse kidney using 83270-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83270-2-RR (DOCK8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using DOCK8 Recombinant antibody (83270-2-RR)

    IF Staining of THP-1 using 83270-2-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using DOCK8 antibody (83270-2-RR, Clone: 230441A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of THP-1 cells using DOCK8 Recombinant antibody (83270-2-RR)

    FC experiment of THP-1 using 83270-2-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using DOCK8 Recombinant antibody (83270-2-RR)

    FC experiment of HeLa using 83270-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83270-2-RR

    Affinity and Kinetic Characterization of 83270-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83270-2-RR against Human DOCK8 were performed. The affinity constant is 10.1 nM.

Tested Applications

Positive WB detected inRamos cells, Raji cells, THP-1 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inHeLa cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83270-2-RR targets DOCK8 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DOCK8 fusion protein Ag34585
Full Name dedicator of cytokinesis 8
Calculated Molecular Weight 2031 aa, 231 kDa
Observed Molecular Weight 239 kDa
GenBank Accession NumberBC019102
Gene Symbol DOCK8
Gene ID (NCBI) 81704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).

Protocols

Product Specific Protocols
WB protocol for DOCK8 antibody 83270-2-RRDownload protocol
IHC protocol for DOCK8 antibody 83270-2-RRDownload protocol
IF protocol for DOCK8 antibody 83270-2-RRDownload protocol
FC protocol for DOCK8 antibody 83270-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols