CoraLite® Plus 488-conjugated DOCK8 Recombinant antibody

DOCK8 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230441A2

Cat no : CL488-83270-2

Synonyms

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Validation Data Gallery

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  IF Staining of THP-1 using CL488-83270-2

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 DOCK8 antibody (CL488-83270-2, Clone: 230441A2 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83270-2 targets DOCK8 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DOCK8 fusion protein Ag34585
• Full Name: dedicator of cytokinesis 8
• Calculated Molecular Weight: 2031 aa, 231 kDa
• Observed Molecular Weight: 239 kDa
• GenBank Accession Number: BC019102
• Gene Symbol: DOCK8
• Gene ID (NCBI): 81704
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).