DOCK8 Recombinant antibody, PBS Only

DOCK8 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230441A2

Cat no : 83270-2-PBS

Synonyms

MRD2, dedicator of cytokinesis 8, 230441A2

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Validation Data Gallery

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  WB analysis using 83270-2-RR (same clone as 83270-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83270-2-RR (DOCK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 83270-2-RR (same clone as 83270-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83270-2-RR (DOCK8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 83270-2-RR (same clone as 83270-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using DOCK8 antibody (83270-2-RR, Clone: 230441A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

• 

  FC experiment of THP-1 using 83270-2-RR (same clone as 83270-2-PBS)

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83270-2-RR (same clone as 83270-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83270-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83270-2-RR against Human DOCK8 were performed. The affinity constant is 10.1 nM.

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Product Information

83270-2-PBS targets DOCK8 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DOCK8 fusion protein Ag34585
• Full Name: dedicator of cytokinesis 8
• Calculated Molecular Weight: 2031 aa, 231 kDa
• Observed Molecular Weight: 239 kDa
• GenBank Accession Number: BC019102
• Gene Symbol: DOCK8
• Gene ID (NCBI): 81704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).