Filter: AllWBIF/ICCFC (Intra)Cytometric bead arrayAffinity

WB analysis using 83227-1-RR (same clone as 83227-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.
WB analysis of MDCK using 83227-1-RR (same clone as 83227-1-PBS)
MDCK cells were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.
IF Staining of U-251 using 83227-1-RR (same clone as 83227-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TCP11L2 antibody (83227-1-RR, Clone: 240096F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.
FC experiment of A431 using 83227-1-RR (same clone as 83227-1-PBS)
1x10^6 A431 cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.
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1x10^6 A431 cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.
FC experiment of SH-SY5Y using 83227-1-RR (same clone as 83227-1-PBS)
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.
×
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP00177-1
Cytometric bead array standard curve of MP00177-1, TCP11L2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83227-1-PBS. Detection antibody: 83227-2-PBS. Standard: Ag11185. Range: 1.25-20 ng/mL.
Affinity and Kinetic Characterization of 83227-1-RR
Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human TCP11L2 were performed. The affinity constant is below 1 pM.

TCP11L2 Recombinant antibody, PBS Only (Capture)

TCP11L2 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83227-1-PBS

Clone No.240096F6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

83227-1-PBS targets TCP11L2 as part of a matched antibody pair:

MP00177-1: 83227-1-PBS capture and 83227-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, canine
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	TCP11L2 fusion protein Ag11185 Predict reactive species
Full Name	t-complex 11 (mouse)-like 2
Calculated Molecular Weight	519 aa, 58 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC033617
Gene Symbol	TCP11L2
Gene ID (NCBI)	255394
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8N4U5
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

TCP11L2 is a protein containing a serine-rich region in its N-terminal region. It is a member of the TCP11 family, which includes SOK1, TCP11L1, and TCP11L2. TCP11L2 has a role in the migration and differentiation of skeletal muscle-derived satellite cells. It has been shown that Tcp11l2 expression gradually increased during the differentiation of bovine muscle-derived satellite cells. (PMID: 32017087)