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ATP1A1 Recombinant antibody

ATP1A1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240160E12

Cat no : 83191-6-RR

Synonyms

Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1A1 Recombinant antibody (83191-6-RR)

    WB analysis using 83191-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83191-6-RR (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP1A1 Recombinant antibody (83191-6-RR)

    IF Staining of HEK-293 using 83191-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP1A1 antibody (83191-6-RR, Clone: 240160E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1A1 Recombinant antibody (83191-6-RR)

    IF Staining of A431 using 83191-6-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-6-RR, Clone: 240160E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HEK-293 cells using ATP1A1 Recombinant antibody (83191-6-RR)

    FC experiment of HEK-293 using 83191-6-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1 Recombinant antibody (83191-6-RR, Clone:240160E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inunboiled A431 cells, unboiled HeLa cells, unboiled RAW 264.7 cells
Positive IF/ICC detected inA431 cells, HEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83191-6-RR is ATP1A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP1A1 fusion protein Ag5763
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Protocols

Product Specific Protocols
WB protocol for ATP1A1 antibody 83191-6-RRDownload protocol
IF protocol for ATP1A1 antibody 83191-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols