CoraLite® Plus 647-conjugated ATP1A1 Recombinant antibody

• 

  IF Staining of A431 using CL647-83191-6

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 647 ATP1A1 antibody (CL647-83191-6, Clone: 240160E12 ) at dilution of 1:200.

ATP1A1 Recombinant Antibody for IF/ICC

Cat No. CL647-83191-6

Clone No.240160E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

---

Tested Applications

• Positive IF/ICC detected in: A431 cells

For optimal WB detection with 83191-6-RR, we do not recommend boiling the sample after lysis.

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-83191-6 targets ATP1A1 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP1A1 fusion protein Ag5763
• Full Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC050359
• Gene Symbol: ATP1A1
• Gene ID (NCBI): 476
• ENSEMBL Gene ID: ENSG00000163399
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05023
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.